Los Guatuzos Wildlife Refuge is located in the south of Lake Nicaragua, within the municipality of San Carlos, Rio San Juan. This natural site is a strip of wetland bordering the nearby country of Costa Rica. Los Guatuzos has an extension of 438 square kilometers consisting of tropical wetlands, rainforest and wildlife refuge. Adventurous travelers can take pleasure in its luxuriant flora and fauna through the magnificent Paputurro River. Here avid nature lovers have unique opportunities to spot all different kind of birds such as northern jacanas, purple gallinules, fasciated-tiger herons, great egrets, green herons, neo-tropic cormorants, anhingas, great kiskadees, and other colorful interesting birds.

Both Los Guatuzos and Papaturro River also provides the opportunity for nature enthusiasts to observe mammals in wildlife like white-faced monkeys, spider monkeys and howler monkeys, actually the loudest monkey in the world. In addition caimans are also seen sunbathing along the river. Reptiles like “green iguanas” are also spotted in lush trees.